Guinea-Bissau will square off with Nigeria in their final 2021 Africa Cup of Nations Group D match at the Roumdé Adjia Stadium in Cameroon on Wednesday.
Kick-off is at 16h00 GMT.
The Djurtus registered a goalless draw in their opening match against Mauritania and that was followed by a 1-0 loss to record African champions Egypt.
The results have left Guinea-Bissau in third pool and with a mathematical chance of advancing into the last 16 if they beat Nigeria.
Baciro Cande, the Guinea-Bissau head coach, will be looking to guide his side to a victory over a much-fancied Nigeria.
Guinea-Bissau looked dangerous going forward against Egypt and Mama Balde had what appeared to be a clean goal scored disallowed by VAR.
Meanwhile, Nigeria booked their place in the knockout stages after beating Sudan 3-1 in Garoua on Saturday.
Nigeria are likely to field a weak team as they impressively defeated Egypt in their opening match and therefore secured not only a spot in the last 16, but also first place in Group D.
This gives the Super Eagles caretaker coach the luxury to rest some of their key players such as Samuel Chukwueze, Wilfred Ndidi and Kelechi Iheanacho to ensure they are fit and fresh for a last 16 clash.
In head-to-head stats, this will be the first competitive meeting of Guinea-Bissau and Nigeria.
The other Group D match in this round sees Egypt take on Sudan earlier on Wednesday evening, also at the Ahmadou Ahidjo Stadium.